How Does Cxbladder Work?
Cxbladder is based on 9 years of scientific research by Pacific Edge, which shows that messenger RNA (mRNA) levels of specific biomarkers are present at higher levels of concentration in patient urine samples that are positive for bladder cancer than in patients who are negative for the disease.
A small sample (2 – 5 mL) of mid stream urine is required for the test. The sample undergoes a precise set of processes to extract and purify the mRNA present in the patient urine sample. The purified RNA is then quantified by a technique called quantitative Reverse Transcription Polymerase Chain Reaction or qRT-PCR. qRT-PCR first involves the conversion of RNA to DNA and then the amplification of that DNA by millions of fold, regulated by a repetitive cycle of temperature adjustment. The resulting millions of copies of DNA are detected by a probe whose fluorescence is directly proportional to the number of copies of DNA present.
In Pacific Edge’s test, Cxbladder, each of the five biomarkers of interest to us is quantified by a different probe and the relationship between the individual biomarkers is determined by a mathematical equation. The calculated outcome provides a measure of the probability of the presence of urinary tract bladder cancer.